Not solely for lesbians, no.
We used to have an official lesbian bar - Rita's Place. It was opened after the first official gay bar in St. John's, Friends, which opened in 1974. Unofficial gay bars, though, or bars with gay nights, opened in the early 1960s.
Now we have two options for lesbians. The main gay bar, Velvet, has ladies nights. And there's also the Station Lounge, which hosts all the lesbian-organized lesbian parties (as a gay man, I often never even know they're happening). Its clientelle is typically lesbian even in between these parties, but not officially so.